Application

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Glypican-4 in 10 µg of mouse whole embryo, mouse E16 brain, and human kidney tissue lysate.

Anti-Glypican-4 Antibody, clone 1C7.2 is an antibody against Glypican-4 for use in Western Blotting.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glypican-4 is a glycosylphosphatidylinositol (GPI)-anchored cell surface heparan sulfate proteoglycan (HSPG) that is encoded by the GPC4 gene in human. Glypican-4 belongs to the family of glypican-related integral membrane proteoglycans (GRIPs) that may be involved in the control of cell division and growth regulation. Deletion of the entire GPC4 gene and the two exons at the 3′ end of the adjacent GPC3 gene has been reported in a patient diagnosed with Simpson-Golabi-Behmel syndrome (SGBS).

Immunogen

GST-tagged recombinant protein corresponding to human Glypican-4.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Mouse monoclonal IgG2bκ in tissue culture supernatant containing 0.05% sodium azide

Unpurified

Quality

Evaluated by Western Blotting in mouse E17 spinal cord tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Glypican-4 in 10 µg of mouse E17 spinal cord tissue lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~62 kDa observed